| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Brachyspira murdochii | ACTTCGGTCATCAGGCTGC | CCCCTTCTCTTGAAGTTCTGC | 60.08 | 58.57 | 174 | 132 |
100.00%
|
100.00%
|
| Primer 2 | Brachyspira murdochii | ATCAGGCTGCCGTAAGCTG | CCCCTTCTCTTGAAGTTCTGC | 60.15 | 58.57 | 165 | 132 |
100.00%
|
100.00%
|
| Primer 3 | Brachyspira murdochii | TCATCAGGCTGCCGTAAGC | CCCCTTCTCTTGAAGTTCTGC | 60.15 | 58.57 | 167 | 132 |
100.00%
|
100.00%
|
| Primer 4 | Brachyspira murdochii | AACTTCGGTCATCAGGCTGC | CCCCTTCTCTTGAAGTTCTGC | 60.67 | 58.57 | 175 | 132 |
100.00%
|
100.00%
|
| Primer 5 | Brachyspira murdochii | CGGCTTTTACATCCGCTGC | AGCCTCATTAGAAAGCGGAGG | 59.94 | 59.86 | 203 | 125 |
100.00%
|
100.00%
|
| Primer 6 | Brachyspira murdochii | ATGTGCTTGCTGCCAACC | GCAAGCGGACATACATGAGC | 58.95 | 59.69 | 259 | 119 |
100.00%
|
100.00%
|
| Primer 7 | Brachyspira murdochii | TGCATGGTACTGGAGGTCC | TCCGCTAACAACTTCACCCC | 58.70 | 59.96 | 188 | 119 |
100.00%
|
100.00%
|
| Primer 8 | Brachyspira murdochii | CGGCTTTTACATCCGCTGC | ACTCTTCTCACAGCCTGAGG | 59.94 | 58.73 | 178 | 118 |
100.00%
|
100.00%
|
| Primer 9 | Brachyspira murdochii | CGGCTTTTACATCCGCTGC | GCCTCATTAGAAAGCGGAGG | 59.94 | 58.41 | 202 | 118 |
100.00%
|
100.00%
|
| Primer 10 | Brachyspira murdochii | TGGGGAGGCAGTTCATTAGC | CGCTGCCGTATTCTTTCACC | 59.74 | 59.63 | 166 | 112 |
100.00%
|
100.00%
|